Zenix™-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. Zenix™-C, uses lay-down monolayer on porous silica as oppose to Zenix of stand-up monolayer, offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation. The 3µm based packing material allows for high resolution and performance separation.